John T. Prince
HHMI Research Specialist (Post-doctoral)
May 2008 – present. University of Colorado (CU), Boulder, Colorado.
- Mass spectrometric identification of transient protein complexes
- Advisor: Natalie Ahn
PhD, Cell and Molecular Biology (Bioinformatics and Computational Biology)
Aug 2001 – May 2008. University of Texas (UT), Center for Systems and Synthetic Biology, Austin, Texas.
B.S., National Merit Scholar, Microbiology (Chemistry Minor)
Dec. 2000. Brigham Young University (BYU), Provo, Utah.
- Graduated Magna Cum Laude (3.89/4.00)
Signal Transduction
May 2008 – present. Dr. Natalie Ahn Lab, CU.
- in vivo cross-linking and mass spectrometry analysis of transient protein-protein interactions
Proteomics & Bioinformatics
Jan. 2002 – May 2008. Dr. Edward Marcotte Lab, UT.
- Computational analysis of mass spectrometry proteomics data
- Maintenance and operation of 2D-LC nanoelectrospray LTQ-Orbitrap and Ion Trap mass spectrometers
- Creator and curator of the Open Proteomics Database (OPD)
Computational Phylogenetics
Mar. – May 2002. Dr. David Hillis Lab, UT.
- Investigation of site-likelihood parameters of Maximum Likelihood models
- Wrote programs and performed computer simulations
Antibody Engineering
Oct. 2001 – Jan. 2002. Dr. George Georgiou Lab, UT.
- Directed evolution of heat stable antibodies by Phage Display
Organic Synthesis
Jun. 1999 – Aug. 2001. Dr. Merritt Andrus Lab, BYU.
- Synthesis of Multi-Drug Resistance reversal agents (cancer research)
- Worked out a mass spectrometry screening assay
- Performed NMR, column and radial chromatography, TLC
Molecular Biology
Fall 2000. Dr. Michael Leavitt Lab, BYU.
- Isolated large, high adherence plasmid in E. coli for design of bacterial interference agents in poultry
- Performed gel electrophoresis, plasmid preps, electroporation, high adherence assay, fluorescence microscopy
Sample bias validation to examine the accuracy of large scale mass spectrometry proteomics peptide identification error estimates. Prince JT and Marcotte EM. Analytical Chemistry (manuscript submitted).
mspire: Mass spectrometry proteomics in Ruby. Prince JT and Marcotte EM. Bioinformatics (manuscript submitted).
Chromatographic alignment of ESI-LC-MS proteomics datasets by bijective interpolated dynamic time warping. Prince JT and Marcotte EM.
Analytical Chemistry. 2006,
78(17), 6140-52.
Mass spectrometry of the M. smegmatis proteome: protein expression levels correlate with function, operons, and codon bias. Wang R, Prince JT, Graham DE, Marcotte EM.
Genome Research. 2005,
15(8), 1118-26.
The need for a public proteomics repository. Prince JT, Carlson MW, Wang R, Lu P, Marcotte EM.
Nature Biotechnology. 2004,
22(4), 471-2.
A fast course filtering method for peptide identification by mass spectrometry. Ramakrishnan SR, Rui M, Nakorchevskiy AA, Prince JT, Willard WS, Xu W, Marcotte EM, Miranker DP.
Bioinformatics. 2006,
22(12), 1524-31.